Promoter isolation
WebFeb 15, 2024 · Isolation and sequence analysis of the CmLOX08 promoter On the basis of the publicly available sequence in the melon genome database ( http://melonomics.net ), we obtained the 2054-bp 5′ flanking sequence of CmLOX08 upstream of the ATG start codon from melon genomic DNA. WebIsolation and Characterization of a Polyubiquitin Gene and Its Promoter Region from Mesembryanthemum crystallinum Muhammad Abul Kalam AZAD, 1Kunio MORITA, Jun-ichi OHNISHI,1 and Shin KORE-EDA1 ;2 y 1Division of Life Sciences, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan 2Comprehensive Analysis …
Promoter isolation
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WebApr 7, 2024 · In the present study, promoter sequences were isolated from four watermelon accessions: Cream of Saskatchewan pale yellow (COS), canary yellow flesh (PI 635597), … WebDec 16, 2024 · Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named PPSP1-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis.
WebAug 5, 2024 · Promoter isolation and promoter::GUS vector construction. The 5ʹ-flanking region upstream of the translation start codon of BnGH17 (BnaC01g21880D) was isolated from line J9712 using sequence-specific primers (Supplementary Table S1) that were based on reference genome sequences (Chalhoub et al., 2014; Song et al., 2024). Web1 hour ago · Nora Forster, a German-born publishing heiress and music promoter who gained fame as the wife of John Lydon – otherwise known as Johnny Rotten of the Sex …
WebFeb 19, 2008 · Isolation of pGC1, a strong guard cell promoter. Guard cell-specific microarray data were analyzed side by side with mesophyll cell-specific microarray data [] to search for strong guard cell promoter candidates with low expression levels in mesophyll cells.Additional guard cell and mesophyll cell microarray experiments were conducted … WebFeb 10, 2024 · Some people who are immunocompromised (have a weakened immune system) are more likely to get sick with COVID-19 or be sick for a longer period. People …
WebA method for easy isolation of promoter fragments from promoter-probe libraries of filamentous fungi Two genomic fragments capable of driving the expression of the hygromycin B resistance gene (hph) were isolated from the phytopathogenic ascomycete Gibberella pulicaris (anamorph Fusarium sambucinum) using a "promoter-probe library" …
WebFeb 15, 2024 · Isolation of the CmLOX08 promoter Genomic DNA was isolated from young melon leaves using a NuClean Plant Genomic DNA Kit (Kangwei Biotech, China) following … esht chicWebTraditionally, molecular cloning is defined as the isolation and amplification of a specific DNA fragment. Most of these fragments are created either by digesting an existing piece of DNA with restriction enzymes or by targeting it via PCR. Short inserts of ~ 100 bp can also be commercially synthesized as complementary single-stranded oligos ... finish tour de franceWebSep 21, 2024 · Background Transgenic technology has become an important technique for crop genetic improvement. The application of well-characterized promoters is essential for developing a vector system for efficient genetic transformation. Therefore, isolation and functional validation of more alternative constitutive promoters to the CaMV35S promoter … finish tour de france 2023The promoters of the selected candidate genes were identified using the available citrus genome sequences (http://citrus.pw.usda.gov/, http://www.phytozome.net/citrus.php, www.citrusgenomedb.org/) and approximately 1–3 kilobase pair region upstream of the translation initiation … See more Public citrus gene expression data available from the Gene Expression Omnibus (GEO) data repository (www.ncbi.nlm.nih.gov/geo) … See more A total of 15–20 independent transgenic tomato lines were generated for each promoter construct using Agrobacterium-mediated transformation and were grown in a greenhouse (See … See more Qualitative analysis of promoter activity was conducted by comparing the intensity of GUSPlus histochemical staining between promoter transgenic lines and controls. Images showing GUSPlus staining in various … See more esht careersWebOct 12, 2013 · For the functional characterization of gene expression, isolation and structural analysis of the region upstream of a gene is required. After the promoter region is isolated, the regions that activate (i.e., enhancers) or repress (i.e., silencers) gene expression are identified by deleting the promoter region [ 4 ]. esht clinical attachmentsWebApr 21, 2016 · Salinity and drought severely affect both plant growth and productivity, making the isolation and characterization of salinity- or drought-inducible promoters suitable for genetic improvement of crop resistance highly desirable. In this study, a 1468-bp sequence upstream of the translation initiation codon ATG of the promoter for ZmGAPP … esht crisis teamWebPromoters mark the start of every transcript and are an important class of regulatory elements. A large, complex protein structure known as the pre-initiation complex (PIC) is … esht bladder and bowel