Splet14. apr. 2024 · The degradation products were mostly identified as 3′- and 5′-shortmers, 3′- and 5′-shortmers with a terminal phosphorothioate or phosphate, internal hydrolysis … Splet29. okt. 2024 · Article. Jul 1991. Rajesh K Gaur. The use of triphenylmethyl (trityl) protecting groups with long alkyl chain substituents, viz., (4-hexadecyloxyphenyl) diphenylmethyl and (4-decyloxyphenyl ...
Oligonucleotide Quality Control by Analytical HPLC
Splet06. sep. 2024 · We developed a liquid chromatography-mass spectrometry method for the identification and semi-quantitation of the metabolites of eluforsen and then applied the … Splet27. okt. 2024 · n-1 shortmers due to reaction failures; n+1 longmers due to single coupling step by successive addition of two molecules of phosphoramidite and ; Residual amount of phosphodiester from incomplete sulfurization and side reaction. facebook chehalem swim team
Oligonucleotide synthesis: Coupling efficiency and quality control
Splet02. apr. 1994 · In every sequencing reaction shortmers would have to be selected from this bank that are suitable to hybridize adjacently on the sequencing template. After their ligation the shortmers would form a long, and hence more specific, primer in the subsequent sequencing reaction. In the present study a computer simulation of large … Splet26. jul. 2024 · More 3’ end shortmers (from N-1 to N-8) were observed after the SPE method . These results indicate that hybridization-based extraction may be characterized by a specific cutoff fragment length for which the capture strand loses affinity and selectivity. So far, this is the main drawback of such an extraction attempt. Splet19. avg. 2011 · Here we are reporting a non-denaturing ion pairing-reversed phase (IP-RP) chromatography method capable of separating optimal duplex (full-length single strands only) from non-optimal duplex variants (containing shortmers, longmers and 2',5'-isomers) using ultraviolet- and mass spectrometric detection. does meiosis makes body cells